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. 1973 Nov;116(2):535-40.
doi: 10.1128/jb.116.2.535-540.1973.

Isolation of temperature-sensitive membrane mutants of Escherichia coli K-12

J F Steenbakkers, J H Broekman, A Kerkenaar, P G de Haan

Isolation of temperature-sensitive membrane mutants of Escherichia coli K-12

J F Steenbakkers et al. J Bacteriol. 1973 Nov.

Abstract

Mutants with impaired biosynthesis of unsaturated fatty acids or altered metabolism of the phospholipids were isolated at a rather high frequency from a set of temperature-sensitive lysis mutants. It is suggested that preselection for the lysis phenotype makes it possible to isolate several kinds of mutants affected in the integrity of the cytoplasmic membrane.

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