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. 1973 Dec;116(3):1107-12.
doi: 10.1128/jb.116.3.1107-1112.1973.

Isolation of Hfr strains from R+ and ColV2+ strains of Escherichia coli and derivation of an R'lac factor by transduction

A Nishimura, Y Nishimura, L Caro

Isolation of Hfr strains from R+ and ColV2+ strains of Escherichia coli and derivation of an R'lac factor by transduction

A Nishimura et al. J Bacteriol. 1973 Dec.

Abstract

Temperature-resistant revertants were isolated from Escherichia coli strains carrying a temperature-sensitive dnaA mutation (initiation of chromosome replication) and either a repressed or a derepressed F-like R factor or a ColV2 factor. Many of the revertants had all the properties of Hfr strains, with a variety of directions and origins of transfer. From one such revertant, episomes carrying the R factor and part of the lac region (R'lac) could be isolated by transduction. This system offers a good selection for Hfr strains produced by integration of various episomes and for the isolation of R' factors.

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References

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