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. 1973;14(3):436-49.
doi: 10.1186/BF03547431.

Bovine mycoplasmas: cultural and biochemical studies. I

H Erno, L Stipkovits

Bovine mycoplasmas: cultural and biochemical studies. I

H Erno et al. Acta Vet Scand. 1973.

Abstract
in English, Swedish

A survey is given of presently known mycoplasmas of bovine source. Media and methods of cultivation are described. Cholesterol dependence, being the basis of division of Mycoplasmatales into Mycoplasmataceae and Acholeplasmataceae, was examined directly and also indirectly employing sensitivity tests to digitonin and sodium polyanethole sulphonate (SPS). All by now recognized species were found to be correctly classified in Mycoplasma and Acholeplasma, respectively. Of the unnamed “serogroups” 2 should be classified in the latter genus, while 6 serogroups were members of the genus Mycoplasma. Correlation was found between the digitonin test and the direct determination of cholesterol requirement, whereas this was not the case with the SPS test.

Der er givet en oversigt over de for øjeblikket kendte mykoplasmer af bovin oprindelse. Medier og dyrkningsbetingelser er angivet. Cholesterolafhængigheden, som danner grundlaget for inddeling af Mycoplasmatales i Mycoplasmataceae og Acholeplasmataceae, er undersøgt dels direkte og dels indirekte ved hjælp af sensitivitetsundersøgelser over for digitonin og natriumpolyanetholsulfonat (NPS). Alle allerede anerkendte arter fandtes at være korrekt placeret i de 2 eneste slægter, Mycoplasma og Acholeplasma. Af de ikke navngivne „serogrupper“ fandtes 2 at tilhøre Acholeplasma, medens 6 serogrupper kunne henføres til Mycoplasma. Der var total overensstemmelse mellem digitonin testen og direkte bestemmelse af cholesterolafhængighed. Dette var ikke tilfældet med NPS testen.

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