Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1973 Dec;12(6):1466-72.
doi: 10.1128/JVI.12.6.1466-1472.1973.

Synthesis of bacteriophage M13-specific proteins in a DNA-dependent cell-free system. II. In vitro synthesis of biologically active gene 5 protein

R N Konings, J Jansen, T Cuypers, J G Schoenmakers

Synthesis of bacteriophage M13-specific proteins in a DNA-dependent cell-free system. II. In vitro synthesis of biologically active gene 5 protein

R N Konings et al. J Virol. 1973 Dec.

Abstract

It is shown that gene 5 protein of bacteriophage M13 is one of the major proteins synthesized in vitro under the direction of M13 replicative-form DNA. By means of DNA-cellulose chromatography, this protein has been purified to homogeneity and its biological characteristics have been compared with those of its native counterpart. Like native gene 5 protein, the purified, in vitro-synthesized protein binds tightly and selectively to single-stranded, but not to double-stranded, DNAs. These results suggest that truly functional gene 5 protein is made in the cell-free system.

PubMed Disclaimer

References

    1. J Ultrastruct Res. 1965 Oct;13(3):318-42 - PubMed
    1. Biochem Biophys Res Commun. 1965 Aug 16;20(4):393-9 - PubMed
    1. Proc Natl Acad Sci U S A. 1965 Aug;54(2):505-13 - PubMed
    1. Biochim Biophys Acta. 1969 Apr 22;179(2):398-407 - PubMed
    1. Cold Spring Harb Symp Quant Biol. 1968;33:473-84 - PubMed

MeSH terms

LinkOut - more resources