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. 1974 Jan;71(1):6-10.
doi: 10.1073/pnas.71.1.6.

Involvement of two protein factors and ATP in in vitro DNA synthesis catalyzed by DNA polymerase 3 of Escherichia coli

J Hurwitz, S Wickner

Involvement of two protein factors and ATP in in vitro DNA synthesis catalyzed by DNA polymerase 3 of Escherichia coli

J Hurwitz et al. Proc Natl Acad Sci U S A. 1974 Jan.

Abstract

The in vitro conversion of single-stranded DNA from bacteriophage fd to duplex structures depends on E. coli RNA polymerase, DNA polymerase III, riboand deoxyribonucleoside triphosphates, Mg(+2), spermidine or DNA-unwinding protein of E. coli, and two additional protein factors, referred to here as Factors I and II. These two factors are also essential for dTMP incorporation catalyzed by DNA polymerase III and dependent on poly(dA).oligo(dT) primer-template. In the latter reaction, there is an absolute dependency on ATP or dATP.

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