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. 1973 Oct;136(2):387-93.
doi: 10.1042/bj1360387.

Cellular reactions of O6-methylguanine, a product of some alkylating carcinogens

C T Miller, P D Lawley, S A Shah

Cellular reactions of O6-methylguanine, a product of some alkylating carcinogens

C T Miller et al. Biochem J. 1973 Oct.

Abstract

Cultures of a purine-requiring mutant of Chinese hamster ovary cells (CHO-104b), randomly bred hamster embryo cells, or Escherichia coli B(s-1) were treated with non-toxic doses of (3)H-labelled O(6)-methylguanine. DNA and RNA were isolated and subjected to enzymic digestion to nucleosides at pH8. The products of digestion were analysed by ion-exchange chromatography on columns of Dowex 50 (NH(4) (+) form) at pH8.9. No (3)H-labelled O(6)-methylguanosine was detected in nucleic acid digests. (3)H-labelled O(6)-methylguanine was O-demethylated yielding [(3)H]guanine in CHO-104b cells. Radioactivity in nucleic acid digests was associated with thymidine, guanosine, deoxyguanosine and an unidentified early-eluting product. Reports of similar unidentified products from nucleic acids labelled with various agents are discussed.

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