New chromatographic form of phenylalanine transfer ribonucleic acid from Escherichia coli growing exponentially in a low-phosphate medium
- PMID: 4595197
- PMCID: PMC246659
- DOI: 10.1128/jb.118.1.209-212.1974
New chromatographic form of phenylalanine transfer ribonucleic acid from Escherichia coli growing exponentially in a low-phosphate medium
Abstract
Reversed-phase chromatography has been used to detect the presence of a new form of phenylalanyl-transfer ribonucleic acid (Phe-tRNA) from Escherichia coli growing exponentially in media containing low but nonlimiting levels of inorganic phosphate. The amount of this extra Phe-tRNA form is greatest in slowly growing cells (0.8 generations/h), and becomes negligible in media supporting a rapid growth rate (2.14 generations/h).
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