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. 1974 Feb;27(2):425-6.
doi: 10.1128/am.27.2.425-426.1974.

Heat injury and recovery of vegetative cells of Clostridium botulinum type E

Heat injury and recovery of vegetative cells of Clostridium botulinum type E

M D Pierson et al. Appl Microbiol. 1974 Feb.

Abstract

Vegetative cells of Clostridium botulinum type E (Tenno) were heated at 40.5 C in a prereduced peptone-yeast extract broth (PY). During the heating period, cell numbers remained essentially constant for 3 h as indicated by roll tube counts in PY agar (PYA); however, injury and recovery from injury were observed when the cells were enumerated using PYA containing either 0.06 or 0.07% bile salts.

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