Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1974 Mar;27(3):480-3.
doi: 10.1128/am.27.3.480-483.1974.

Tryptic soy bile-kanamycin test for the indentification of Bacteroides fragilis

V Vargo, M Korzeniowski, E H Spaulding

Tryptic soy bile-kanamycin test for the indentification of Bacteroides fragilis

V Vargo et al. Appl Microbiol. 1974 Mar.

Abstract

A simple and practical test for the identification of Bacteroides fragilis is described. It utilizes two well-known properties of this species, i.e., stimulation of growth by bile and resistance to kanamycin. The test media are a tryptic-soy bile agar plate and a supplemented blood agar plate on which a kanamycin 1,000-mug/ml disk is placed. Incubation is for 24 h at 37 C in GasPak. The results of screening 190 strains, mostly clinical isolates, indicate that B. fragilis can be easily and reliably distinguished from other Bacteroides and from Fusobacterium species by its growth on tryptic-soy bile agar and resistance to kanamycin.

PubMed Disclaimer

References

    1. J Bacteriol. 1967 Nov;94(5):1443-50 - PubMed
    1. J Infect Dis. 1969 Jun;119(6):641-9 - PubMed
    1. Mayo Clin Proc. 1970 Apr;45(4):256-64 - PubMed
    1. Appl Microbiol. 1970 Nov;20(5):737-41 - PubMed
    1. Appl Microbiol. 1971 Jan;21(1):13-20 - PubMed

LinkOut - more resources