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. 1974 Jun;27(6):1170-1.
doi: 10.1128/am.27.6.1170-1171.1974.

Immunofluorescent identification of mycoplasma colonies grown on agar-covered glass slides

H D Lehmkuhl, M L Frey

Immunofluorescent identification of mycoplasma colonies grown on agar-covered glass slides

H D Lehmkuhl et al. Appl Microbiol. 1974 Jun.

Abstract

Mycoplasma colonies were grown on microscope slides overlaid with solid medium. After the slides were dried, washed, acetone fixed, and stained with fluorescein-conjugated antisera, specific immunofluorescence was easily observed against a dark background.

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