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. 1973 Oct;4(4):392-5.
doi: 10.1128/AAC.4.4.392.

Variables influencing susceptibility testing of Cryptococcus neoformans to 5-fluorocytosine

E R Block, A E Jennings, J E Bennett

Variables influencing susceptibility testing of Cryptococcus neoformans to 5-fluorocytosine

E R Block et al. Antimicrob Agents Chemother. 1973 Oct.

Abstract

The minimum inhibitory concentration (MIC) of 5-fluorocytosine (5-FC) was determined for 65 isolates of Cryptococcus neoformans by using a twofold serial tube dilution method. The MIC was profoundly influenced by incubation temperature, inoculum size, and duration of incubation. By using a standard set of test conditions, 100% of 49 pretreatment isolates of C. neoformans were susceptible to 10 mug of 5-FC per ml or less, and 9 (56%) of 16 isolates recovered during or after 5-FC therapy were massively drug resistant (MIC > 320 mug/ml). With the standard test conditions recommended here, the tube dilution method was found to be both accurate and reproducible, and the results correlated with the treatment status of patients.

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References

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