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. 1973 Nov;4(5):569-73.
doi: 10.1128/AAC.4.5.569.

Rapid gentamicin bioassay using a multiple-antibiotic-resistant strain of Klebsiella pneumoniae

M E Lund, D J Blazevic, J M Matsen

Rapid gentamicin bioassay using a multiple-antibiotic-resistant strain of Klebsiella pneumoniae

M E Lund et al. Antimicrob Agents Chemother. 1973 Nov.

Abstract

A rapid bioassay for gentamicin levels in serum was developed by using a strain of Klebsiella pneumoniae that has multiple resistance to antibiotics. Assays were comparable when performed on either small or large petri plates, and results were available in 2 to 4 h. Studies showed an overall recovery of 97.6% for gentamicin alone or 104.5% in the presence of commonly used antibiotics. The procedure can be performed without the need to inactivate other antibiotics that may be present in the serum sample.

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