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. 1974 Apr;76(4):625-31.
doi: 10.1093/genetics/76.4.625.

Some improved methods in P22 transduction

P E Hartman

Some improved methods in P22 transduction

P E Hartman. Genetics. 1974 Apr.

Abstract

Recent refinements simplify methods for P22 transduction in Salmonella and allow improved recovery of phage-free transductional clones. The methods include use of: integration- and lysis-defective phage mutants, heat-killed bacteria to eliminate free phage, direct plating of phage and bacteria, replica-plating for detection of phage content of individual clones, improved broth for phage growth, and procurement of high titer phage from P22 lysogens.

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References

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