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. 1974 May;71(5):1743-7.
doi: 10.1073/pnas.71.5.1743.

Replication and transcription of eukaryotic DNA in Escherichia coli

J F Morrow, S N Cohen, A C Chang, H W Boyer, H M Goodman, R B Helling

Replication and transcription of eukaryotic DNA in Escherichia coli

J F Morrow et al. Proc Natl Acad Sci U S A. 1974 May.

Abstract

Fragments of amplified Xenopus laevis DNA, coding for 18S and 28S ribosomal RNA and generated by EcoRI restriction endonuclease, have been linked in vitro to the bacterial plasmid pSC101; and the recombinant molecular species have been introduced into E. coli by transformation. These recombinant plasmids, containing both eukaryotic and prokaryotic DNA, replicate stably in E. coli. RNA isolated from E. coli minicells harboring the plasmids hybridizes to amplified X. laevis rDNA.

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References

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