Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1974 Jul;119(1):270-81.
doi: 10.1128/jb.119.1.270-281.1974.

Macromolecular composition during steady-state growth of Escherichia coli B-r

P P Dennis, H Bremer

Macromolecular composition during steady-state growth of Escherichia coli B-r

P P Dennis et al. J Bacteriol. 1974 Jul.

Abstract

By using Escherichia coli B/r, the cellular amounts of ribonucleic acid (RNA) and protein were determined as a function of the steady-state growth rate (0.67 to 2.40 doublings per h) by a method which combines measurements of the RNA to deoxyribonucleic acid (DNA) ratio and the differential rate of ribosomal protein synthesis with the Cooper and Helmstetter theory of DNA replication. The results indicate that the ratios RNA/DNA, RNA/protein, and protein/DNA give linear relationships with the growth rate (above 1.2 doublings per h), whereas RNA/cell and protein/cell show a more complex growth rate dependency. The significance of these relationships is discussed. Finally, a detailed description of the growth parameters and composition of E. coli B/r is presented.

PubMed Disclaimer

References

    1. Genetics. 1965 Oct;52(4):747-57 - PubMed
    1. J Mol Biol. 1966 Jul;18(2):308-20 - PubMed
    1. J Mol Biol. 1967 Jul 14;27(1):41-55 - PubMed
    1. J Mol Biol. 1968 Feb 14;31(3):507-18 - PubMed
    1. J Mol Biol. 1968 Feb 14;31(3):519-40 - PubMed

LinkOut - more resources