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. 1974 Aug;119(2):494-9.
doi: 10.1128/jb.119.2.494-499.1974.

Mapping of a mutation, polB100, affecting deoxyribonucleic acid polymerase II in Escherichia coli K-12

J L Campbell, H Shizuya, C C Richardson

Mapping of a mutation, polB100, affecting deoxyribonucleic acid polymerase II in Escherichia coli K-12

J L Campbell et al. J Bacteriol. 1974 Aug.

Abstract

Direct assay for deoxyribonucleic acid polymerase II in extracts has been used to screen recombinants for the polB allele in Hfr x F(-) crosses, F-ductants in episome transfer, and transductants in generalized transduction by phage P1. The polB gene is located at 2 min on the Escherichia coli linkage map; it is 39 to 64% co-transducible with leu. A mutant, E. coli polA1 polB100 polC (ts), deficient in deoxyribonucleic acid polymerases I and II and having a thermolabile deoxyribonucleic acid polymerase III, has been prepared by the P1-mediated cross: P1 (HMS85 polB100) x E. coli BT1026 polA1 polC (ts) leu(-).

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