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. 1974 Aug;28(2):169-71.
doi: 10.1128/am.28.2.169-171.1974.

Radiometric method for the detection of coliform organisms in water

U Bachrach, Z Bachrach

Radiometric method for the detection of coliform organisms in water

U Bachrach et al. Appl Microbiol. 1974 Aug.

Abstract

A new radiometric method for the detection of coliform bacteria in water has been described. The method is based on the release of (14)CO(2) from [(14)C]lactose by bacteria suspended in growth medium and incubated at 37 C. The evolved (14)CO(2) is trapped by hyamine hydroxide and counted in a liquid scintillation spectrometer. The method permits the detection of 1 to 10 organisms within 6 h of incubation. Coliform bacteria suspended in water for several days recover from starvation and may be quantitated by the proposed method. Bacteria from water samples may also be concentrated by filtration through membrane filters and detected by the radiometric assay.

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References

    1. Appl Microbiol. 1973 Jun;25(6):1011-2 - PubMed
    1. Antimicrob Agents Chemother. 1972 Nov;2(5):360-66 - PubMed
    1. Appl Microbiol. 1971 Nov;22(5):846-9 - PubMed
    1. Appl Microbiol. 1972 Jan;23(1):198-9 - PubMed
    1. Science. 1962 Oct 12;138(3537):114-21 - PubMed

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