Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1974 Mar;26(3):549-61.

Recovery of immunoglobulin removed from lymphocytic surfaces by proteolysis

R P Eady, D W Hough, P J Kilshaw, G T Stevenson

Recovery of immunoglobulin removed from lymphocytic surfaces by proteolysis

R P Eady et al. Immunology. 1974 Mar.

Abstract

The removal of immunoglobulin from the surfaces of human lymphocytes by limited proteolysis has been monitored by radioimmunoassay of the supernatants and immunolabelling of the residual membrane.

Sublethal amounts of papain or trypsin can rapidly remove at least the immunoglobulin Fab regions from both normal and leukaemic lymphocytes.

The Fabμ regions survive the high enzyme: substrate ratios prevailing, and thus are recoverable from the digests.

PubMed Disclaimer

References

    1. J Exp Med. 1972 Jul 1;136(1):191-6 - PubMed
    1. N Engl J Med. 1972 Aug 10;287(6):272-6 - PubMed
    1. J Exp Med. 1972 Aug 1;136(2):409-14 - PubMed
    1. J Exp Med. 1972 Oct 1;136(4):676-96 - PubMed
    1. Blood. 1972 Dec;40(6):777-94 - PubMed

LinkOut - more resources