doi: 10.1128/jb.120.1.407-415.1974.
Isolation and characterization of an Escherichia coli K-12 mutant with a temperature-sensitive recA- phenotype
- PMID: 4607624
- PMCID: PMC245777
- DOI: 10.1128/jb.120.1.407-415.1974
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Isolation and characterization of an Escherichia coli K-12 mutant with a temperature-sensitive recA- phenotype
J Bacteriol.
1974 Oct.
Abstract
A mutation that causes a temperature-sensitive RecA(-) phenotype was identified in a derivative of a PolA(-) strain that failed to grow at high temperature. The mutant allele (recA200) was shown to be linked to cysC, conferred a sharply temperature-sensitive, ultraviolet-sensitive Rec(-) phenotype in the range 35 to 42 C, and in crosses failed to show complementation at 42 C with Hfr's that transferred recA(-). Double mutants that carried both recA200 and polA were examined for ability to grow and synthesize DNA at restrictive temperatures.
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