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. 1974 Oct;28(4):618-22.
doi: 10.1128/am.28.4.618-622.1974.

Identification of Salmonella with the O-1 bacteriophage

S Welkos, M Schreiber, H Baer

Identification of Salmonella with the O-1 bacteriophage

S Welkos et al. Appl Microbiol. 1974 Oct.

Abstract

The O-1 bacteriophage test of Cherry et al. (1954) for the presumptive identification of salmonellae in the diagnostic laboratory was investigated. A phage lysate with a titer of 10(12) plaque-forming units per ml was found to be optimal. This preparation lysed 98.2% of Salmonella strains tested, while maintaining its high specificity for salmonellae. Gram-negative organisms other than salmonellae were resistant to the O-1 phage; however, 5.9% of Escherichia coli strains tested were susceptible. The O-1 phage test is a simple, rapid, inexpensive, sensitive, and specific procedure for the identification of salmonellae in the diagnostic laboratory. A presumptive identification is obtained 1 day earlier than with conventional biochemical tests.

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References

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