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. 1974 Oct;71(10):3854-7.
doi: 10.1073/pnas.71.10.3854.

Relaxation complexes of plasmids ColE1 and ColE2: unique site of the nick in the open circular DNA of the relaxed complexes

M A Lovett, D G Guiney, D R Helinski

Relaxation complexes of plasmids ColE1 and ColE2: unique site of the nick in the open circular DNA of the relaxed complexes

M A Lovett et al. Proc Natl Acad Sci U S A. 1974 Oct.

Abstract

The product of the induced relaxation of supercoiled DNA-protein relaxation complexes of colicinogenic factors E1 (ColE1) and E2 (ColE2) is an open circular DNA molecule with a strand-specific nick. Cleavage of the open circular DNA of each relaxed complex with the EcoRI restriction endonuclease demonstrates that the single-strand break is at a unique position. The site of the single-strand break in the relaxed ColE1 complex is approximately the same distance from the EcoRI cleavage site as the origin of ColE1 DNA replication.

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