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. 1974 Oct;71(10):4149-53.
doi: 10.1073/pnas.71.10.4149.

Cell-free synthesis of a specific lipoprotein of the Escherichia coli outer membrane directed by purified messenger RNA

A Hirashima, S Wang, M Inouye

Cell-free synthesis of a specific lipoprotein of the Escherichia coli outer membrane directed by purified messenger RNA

A Hirashima et al. Proc Natl Acad Sci U S A. 1974 Oct.

Abstract

A specific lipoprotein of the E. coli outer membrane has been synthesized in a cell-free system directed by purified messenger RNA. The mRNA for the lipoprotein was purified as 7S RNA about 250-fold from exponentially growing cells. Protein synthesis of the cell-free system was totally dependent upon the addition of the purified mRNA. The product of the cell-free system was identified as the specific lipoprotein by immunoprecipitation and by peptide mapping.

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References

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    1. J Biol Chem. 1972 Dec 25;247(24):8154-9 - PubMed
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    1. Nature. 1973 Apr 6;242(5397):405-7 - PubMed
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