doi: 10.1073/pnas.71.10.4149.
Cell-free synthesis of a specific lipoprotein of the Escherichia coli outer membrane directed by purified messenger RNA
- PMID: 4610570
- PMCID: PMC434347
- DOI: 10.1073/pnas.71.10.4149
Item in Clipboard
Cell-free synthesis of a specific lipoprotein of the Escherichia coli outer membrane directed by purified messenger RNA
Proc Natl Acad Sci U S A.
1974 Oct.
Abstract
A specific lipoprotein of the E. coli outer membrane has been synthesized in a cell-free system directed by purified messenger RNA. The mRNA for the lipoprotein was purified as 7S RNA about 250-fold from exponentially growing cells. Protein synthesis of the cell-free system was totally dependent upon the addition of the purified mRNA. The product of the cell-free system was identified as the specific lipoprotein by immunoprecipitation and by peptide mapping.
Similar articles
-
Amino acid sequence for the peptide extension on the prolipoprotein of the Escherichia coli outer membrane.Proc Natl Acad Sci U S A. 1977 Mar;74(3):1004-8. doi: 10.1073/pnas.74.3.1004. Proc Natl Acad Sci U S A. 1977. PMID: 322142 Free PMC article.
-
Purification of the messenger ribonucleic acid for the lipoprotein of the Escherichia coli outer membrane.Biochemistry. 1979 Oct 2;18(20):4270-7. doi: 10.1021/bi00587a002. Biochemistry. 1979. PMID: 114211
-
A new form of structural lipoprotein of outer membrane of Escherichia coli.J Biol Chem. 1977 Apr 10;252(7):2324-30. J Biol Chem. 1977. PMID: 321456
-
[Messenger RNA for the lipoprotein of the Escherichia coli outer membrane (author's transl)].Seikagaku. 1978 Jul;50(7):495-500. Seikagaku. 1978. PMID: 361903 Review. Japanese. No abstract available.
-
Structure and biosynthesis of functionally defined areas of the Escherichia coli outer membrane.Ann N Y Acad Sci. 1974 May 10;235(0):66-82. doi: 10.1111/j.1749-6632.1974.tb43257.x. Ann N Y Acad Sci. 1974. PMID: 4605152 Review. No abstract available.
Cited by
-
Insertion of a MalE beta-galactosidase fusion protein into the envelope of Escherichia coli disrupts biogenesis of outer membrane proteins and processing of inner membrane proteins.J Bacteriol. 1982 Oct;152(1):133-9. doi: 10.1128/jb.152.1.133-139.1982. J Bacteriol. 1982. PMID: 6749803 Free PMC article.
-
Amino acid sequence for the peptide extension on the prolipoprotein of the Escherichia coli outer membrane.Proc Natl Acad Sci U S A. 1977 Mar;74(3):1004-8. doi: 10.1073/pnas.74.3.1004. Proc Natl Acad Sci U S A. 1977. PMID: 322142 Free PMC article.
-
Novel mutation that causes a structural change in a lipoprotein in the outer membrane of Escherichia coli.J Bacteriol. 1976 Sep;127(3):1494-1501. doi: 10.1128/jb.127.3.1494-1501.1976. J Bacteriol. 1976. PMID: 783146 Free PMC article.
-
Synthesis and processing of an Escherichia coli alkaline phosphatase precursor in vitro.Proc Natl Acad Sci U S A. 1977 Apr;74(4):1440-4. doi: 10.1073/pnas.74.4.1440. Proc Natl Acad Sci U S A. 1977. PMID: 323853 Free PMC article.
-
Extracellular peptide production in Escherichia coli by inducible downregulation of lipoprotein Lpp via MicL sRNA.Appl Microbiol Biotechnol. 2025 Jun 4;109(1):136. doi: 10.1007/s00253-025-13524-z. Appl Microbiol Biotechnol. 2025. PMID: 40468089 Free PMC article.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
