doi: 10.1093/nar/6.6.2041.
Rapid synthesis of oligodeoxyribonucleotides on a grafted polymer support
- PMID: 461181
- PMCID: PMC327835
- DOI: 10.1093/nar/6.6.2041
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Rapid synthesis of oligodeoxyribonucleotides on a grafted polymer support
Nucleic Acids Res.
1979.
Abstract
Rapid synthesis of oligodeoxyribonucleotides is described by a solid phase method. Polysterene grafted on the surface of polytetrafluoroethylene has been found to be an adequate support. The properties of the support were studied in the synthesis of a number of oligonucleotides 6 to 15 nucleotides long. A flow-type semi-automatic synthesizer has been used. Each nucleotide addition involves seven steps and takes 8 hours. Oligonucleotide isolated and purified by ion-exchange chromatography amounts to 1-3 mumol per gram of support.
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