doi: 10.1128/jb.120.3.1223-1226.1974.
Construction of phi80 dhis carrying Salmonella typhimurium histidine operon mutations
- PMID: 4612009
- PMCID: PMC245904
- DOI: 10.1128/jb.120.3.1223-1226.1974
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Construction of phi80 dhis carrying Salmonella typhimurium histidine operon mutations
J Bacteriol.
1974 Dec.
Abstract
Starting with a previously isolated F'his episome and phi80 dhis imm(lambda) cI857 transducing phage, we have constructed recombinant elements bearing previously isolated his mutations from Salmonella typhimurium. These phages were constructed as sources of deoxyribonucleic acid for in vitro biochemical experiments on gene regulation. The manipulation of genes between S. typhimurium and Escherichia coli described here may be useful in studying other S. typhimurium operons.
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