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. 1974 Nov;71(11):4556-60.
doi: 10.1073/pnas.71.11.4556.

Utilization of two distinct modes of replication by a hybrid plasmid constructed in vitro from separate replicons

K Timmis, F Cabello, S N Cohen

Utilization of two distinct modes of replication by a hybrid plasmid constructed in vitro from separate replicons

K Timmis et al. Proc Natl Acad Sci U S A. 1974 Nov.

Abstract

A hybrid plasmid, pSC134, that codes for two distinct sets of replication functions has been constructed in vitro by ligation of EcoRI endonuclease-cleaved pSC101 and Col E1 plasmid replicons, and has been introduced into Escherichia coli by transformation. The replication properties of the pSC134 plasmid in DNA polymerase I-defective mutants or in the presence of chloramphenicol indicate that this hybrid plasmid can utilize the functionally distinct modes of replication specified by both of its parent replicons.

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References

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