Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1974 Nov;120(2):805-14.
doi: 10.1128/jb.120.2.805-814.1974.

The rep mutation. III. Altered structure of the replicating Escherichia coli chromosome

H E Lane, D T Denhardt

The rep mutation. III. Altered structure of the replicating Escherichia coli chromosome

H E Lane et al. J Bacteriol. 1974 Nov.

Abstract

The rep gene function of Escherichia coli is essential for the replication of P2 and phiX174 double-stranded deoxyribonucleic acid (DNA). Compared with isogenic rep(+) strains, rep mutants show the following characteristics: larger cell size, more DNA per cell, and a slightly lower DNA/mass ratio. The replicating rep chromosomes show a steeper gradient of marker frequencies and contain more replicating forks per chromosome. The nucleoid body of rep mutants sediments faster and contains more DNA. We deduce that the rep function is required for the "normal" replication of the E. coli chromosome and that in its absence the E. coli chromosome replicates in an altered manner, perhaps involving slower-moving replicating forks.

PubMed Disclaimer

References

    1. J Mol Biol. 1972 Oct 14;70(3):549-66 - PubMed
    1. J Mol Biol. 1970 Mar 14;48(2):329-38 - PubMed
    1. J Mol Biol. 1971 Aug 28;60(1):65-74 - PubMed
    1. Proc Natl Acad Sci U S A. 1971 Jan;68(1):6-9 - PubMed
    1. Virology. 1972 Aug;49(2):486-96 - PubMed

LinkOut - more resources