Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1974 Sep;141(3):701-13.
doi: 10.1042/bj1410701.

A mass-spectrometric sequence study of the enzyme ribitol dehydrogenase from Klebsiella aerogenes

H R Morris, D H Williams, G G Midwinter, B S Hartley

A mass-spectrometric sequence study of the enzyme ribitol dehydrogenase from Klebsiella aerogenes

H R Morris et al. Biochem J. 1974 Sep.

Abstract

The first detailed results of the application of a low-resolution mixture analysis approach to the sequence analysis of an enzyme, ribitol dehydrogenase, are given. Examples of the interpretation of the spectra of peptide mixtures derived from this protein are described. Evidence for new fragmentation patterns observed is reported, together with an explanation of the generation of ambiguous sequences by use of a low-specificity enzyme, thermolysin. The overall sequencing strategy evolved is assessed.

PubMed Disclaimer

References

    1. Biochem J. 1974 Feb;137(2):409-11 - PubMed
    1. Biochem J. 1974 Sep;141(3):693-700 - PubMed
    1. Nature. 1968 Aug 3;219(5153):498-9 - PubMed
    1. Nature. 1966 Jul 23;211(5047):361-6 - PubMed
    1. Biochem Biophys Res Commun. 1973 Mar 5;51(1):247-55 - PubMed