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. 1979 Mar;23(3):547-59.

Purification of rabbit antispermine antiserum by affinity chromatography

F Bartos, D Bartos, R A Campbell, D P Grettie, P McDaniel, M Davis, P Smejtek
  • PMID: 461975

Purification of rabbit antispermine antiserum by affinity chromatography

F Bartos et al. Res Commun Chem Pathol Pharmacol. 1979 Mar.

Abstract

Immunoglobulins G were isolated from a crude rabbit antispermine antiserum using a protein A-Sepharose CL-4B column. Affinity chromatography coupling spermine to the Sepharose matrix was employed for separation of antispermine antibodies from these immunoglobulins. Four fractions of antispermine antibodies were isolated by stepwise elution with solutions at pH range from 4 to 1. Binding constants were determined and cross-reactivity with other polyamines was tested for each fraction of antibodies. Highly specific antispermine antibodies were found in fraction IV.

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