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Comparative Study
. 1972 Feb;23(2):285-8.
doi: 10.1128/am.23.2.285-288.1972.

Rapid extraction method with pronase B for grouping beta-hemolytic streptococci

G M Ederer, M M Herrmann, R Bruce, J M Matsen, S S Chapman
Comparative Study

Rapid extraction method with pronase B for grouping beta-hemolytic streptococci

G M Ederer et al. Appl Microbiol. 1972 Feb.

Abstract

The enzyme, Pronase B, was used to extract the C carbohydrates of betahemolytic streptococci for serological grouping. Reference strains of streptococci, groups A through N, were accurately grouped by using this extraction method. Of the beta-hemolytic streptococci isolated from patients' cultures, 1,197 were classified as either group A or not group A by this method. There was 100% correlation with the reference Lancefield method. In contrast, false reactions occurred with the presumptive bacitracin disc method in 4.1% of the group A and 12.7% of the non-group A cultures. The Pronase method proved simple, accurate, and readily adaptable to the clinical laboratory routine.

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