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. 1972 Jul;24(1):22-5.
doi: 10.1128/am.24.1.22-25.1972.

Indirect hemagglutination test for chlamydial antibodies

Indirect hemagglutination test for chlamydial antibodies

V J Lewis et al. Appl Microbiol. 1972 Jul.

Abstract

An indirect hemagglutination (IHA) test is described for chlamydial antibodies in psittacosis diagnostic sera; for this test tanned sheep erythrocytes sensitized with a deoxycholate extract of Chlamydia psittaci grown in Vero cell monolayers were used. Adaptation of the IHA test to the Microtiter system decreased sensitivity; nevertheless, the Microtiter-IHA test was more sensitive than the complement fixation test. Lymphogranuloma venereum antibodies also were detected by using antigen extracted from C. psittaci.

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