Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1972 Sep;10(3):328-39.
doi: 10.1128/JVI.10.3.328-339.1972.

Selection and preliminary characterization of temperature-sensitive mutants of type 5 adenovirus

Selection and preliminary characterization of temperature-sensitive mutants of type 5 adenovirus

M J Ensinger et al. J Virol. 1972 Sep.

Abstract

Eight temperature-sensitive (ts) mutants that replicate normally at 32 C but poorly, if at all, at 39.5 C have been isolated from mutagenized stocks of a wild-type strain of type 5 adenovirus. Three mutagens were employed: nitrous acid, hydroxylamine, and nitrosoguanidine. Ts mutants were isolated from mutagenized viral stocks with frequencies between 0.01 and 0.1%. All eight mutants had reversion frequencies of 10(-5) or less. Complementation experiments in doubly infected cultures at the nonpermissive temperature separated the mutants into three nonoverlapping complementation groups. Complementation yields ranged from a 2.3- to a 3,000-fold increase over the sums of the yields from the two singly infected controls. Genetic recombination was also demonstrated; approximate recombination frequencies ranged from 0.1 to 15%. Preliminary biochemical and immunological characterization of the mutants indicated that: (i) the single mutant in complementation group I did not replicate its deoxyribonucleic acid (DNA) or synthesize late proteins at the nonpermissive temperature but did inhibit host DNA synthesis to 25% of an uninfected control; (ii) the four group II mutants replicated viral DNA, shut off host DNA synthesis, synthesized penton base and fiber, but did not synthesize immunologically detectable hexon; the three mutants in complementation group III synthesized viral DNA, shut off host DNA synthesis, and made immunologically reactive capsid proteins (hexon, penton base, and fiber).

PubMed Disclaimer

References

    1. Virology. 1966 Apr;28(4):782-3 - PubMed
    1. Virology. 1966 Oct;30(2):204-13 - PubMed
    1. Virology. 1968 May;35(1):41-9 - PubMed
    1. Virology. 1968 Sep;36(1):115-25 - PubMed
    1. Virology. 1968 Sep;36(1):126-36 - PubMed

MeSH terms

LinkOut - more resources