doi: 10.1073/pnas.69.10.3049.
Hybridization of variants of chloramphenicol acetyltransferase specified by fi + and fi - R factors
- PMID: 4628098
- PMCID: PMC389704
- DOI: 10.1073/pnas.69.10.3049
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Hybridization of variants of chloramphenicol acetyltransferase specified by fi + and fi - R factors
Proc Natl Acad Sci U S A.
1972 Oct.
Abstract
An fi(-) type of R factor for transferable chloramphenicol resistance carries the structural gene for a novel species of chloramphenicol acetyltransferase (EC 2.3.1.99). The enzyme associated with the fi(-) plasmid is distinct from that described for fi(+) R factors. The fi(+)- and fi(-)-related variants of chloramphenicol acetyltransferase were hybridized in vivo and in vitro. Both techniques yielded only a single symmetrical heteromeric species rather than the three (A(3)B(1), A(2)B(2), and A(1)B(3)) hybrids predicted from the tetrameric (identical monomers) structure of chloramphenicol acetyltransferase.
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