Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1972 Nov;69(11):3211-4.
doi: 10.1073/pnas.69.11.3211.

In vitro repair of x-irradiated DNA extracted from Bacillus subtilis deficient in polymerase I

P J Laipis, A T Ganesan

In vitro repair of x-irradiated DNA extracted from Bacillus subtilis deficient in polymerase I

P J Laipis et al. Proc Natl Acad Sci U S A. 1972 Nov.

Abstract

DNA extracted from x-irradiated cells of a mutant of Bacillus subtilis deficient in DNA polymerase I has greatly reduced biological activity. This DNA, which has many single-strand nicks, can be repaired in vitro with purified DNA polymerase I and DNA ligase or with lysates of wild-type B. subtilis cells, which restore biological activity and increase the single-strand molecular weight. Lysates of polymerase I-deficient B. subtilis cells cannot repair such irradiated DNA until purified DNA polymerase I or lysate from wild-type cells is added to the deficient lysate.

PubMed Disclaimer

References

    1. J Bacteriol. 1961 May;81(5):741-6 - PubMed
    1. J Gen Physiol. 1966 Jul;49(6):233-58 - PubMed
    1. J Biol Chem. 1962 Mar;237:829-33 - PubMed
    1. J Biol Chem. 1969 Jun 10;244(11):2996-3008 - PubMed
    1. Biochem J. 1956 Feb;62(2):315-23 - PubMed

LinkOut - more resources