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. 1973 Jan;70(1):103-7.
doi: 10.1073/pnas.70.1.103.

Inhibition of a DNA polymerase from Bacillus subtilis by hydroxyphenylazopyrimidines

K B Gass, R L Low, N R Cozzarelli

Inhibition of a DNA polymerase from Bacillus subtilis by hydroxyphenylazopyrimidines

K B Gass et al. Proc Natl Acad Sci U S A. 1973 Jan.

Abstract

The antimicrobial agent, 6-(p-hydroxyphenylazo)-uracil, specifically inhibits DNA polymerase III of Bacillus subtilis with a K(i) of less than 1 muM. The inhibition requires prior reduction of the drug, is reversible, and is competitive with dGTP. High amounts of dATP prevent inhibition by the closely related drug, 6-(p-hydroxyphenylazo)-isocytosine. A model is presented in which the inhibitors base-pair with the template while part of a ternary complex with the enzyme. These results imply that DNA polymerase III of B. subtilis is necessary for chromosomal replication.

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