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. 1973 Jan;70(1):103-7.
doi: 10.1073/pnas.70.1.103.

Inhibition of a DNA polymerase from Bacillus subtilis by hydroxyphenylazopyrimidines

K B Gass, R L Low, N R Cozzarelli

Inhibition of a DNA polymerase from Bacillus subtilis by hydroxyphenylazopyrimidines

K B Gass et al. Proc Natl Acad Sci U S A. 1973 Jan.

Abstract

The antimicrobial agent, 6-(p-hydroxyphenylazo)-uracil, specifically inhibits DNA polymerase III of Bacillus subtilis with a K(i) of less than 1 muM. The inhibition requires prior reduction of the drug, is reversible, and is competitive with dGTP. High amounts of dATP prevent inhibition by the closely related drug, 6-(p-hydroxyphenylazo)-isocytosine. A model is presented in which the inhibitors base-pair with the template while part of a ternary complex with the enzyme. These results imply that DNA polymerase III of B. subtilis is necessary for chromosomal replication.

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References

    1. J Biol Chem. 1966 Jul 10;241(13):3083-9 - PubMed
    1. Fed Proc. 1964 Sep-Oct;23:940-5 - PubMed
    1. J Biol Chem. 1968 Feb 10;243(3):627-38 - PubMed
    1. J Biol Chem. 1969 Jun 10;244(11):2996-3008 - PubMed
    1. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1454-61 - PubMed

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