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. 1972;13(2):251-9.
doi: 10.1186/BF03548579.

The production and some properties of the brown pigment of Aeromonas liquefaciens

K Aurstad, H K Dahle

The production and some properties of the brown pigment of Aeromonas liquefaciens

K Aurstad et al. Acta Vet Scand. 1972.

Abstract
in English, German

The optimum conditions for pigment production by Ae. liquefaciens was found to be 30 °C and aerobic incubation, and the properties of the pigment are shown to be similar to those of the synthetic dopa-melanin referred to. The enzyme phenol oxidase was demonstrated in the culture filtrates, but not in extracts of disintegrated cells. As precursors for the pigment production, which was shown to be strictly pH dependent, DL-tyrosine, DL-dopa and catechol were used with success, while numerous other amino acids failed. The significance of the pigment as a criterium for the identification of strains which are pathogenic to certain animals or fishes, is not known.

De optimale atmosfære- og temperaturforhold for pigmentproduksjon hos Ae. liquefaciens ble funnet å være aerob inkubasjon ved 30 °G, og pigmentets egenskaper tilsvarer egenskapene til det syntetiske dopamelanin som det refereres til. Enzymet phenoloxidase ble påvist i kulturfiltrat av Ae. liquefaciens, men ikke i ekstrakt av knuste bakterieceller. Som byggesteiner for pigment-produksjonen ble DL-tyrosin, DL-dopa og catechol brukt med suksess, mens flere andre aminosyrer ikke induserte pigmentproduksjonen. Betydningen av pigmentet som kriterium for identifikasjon av stammer som er patogene for enkelte dyr eller fisk er ikke kjent.

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