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. 1972 Nov;13(6):824-6.

A simple lipase assay using trichloroacetic acid

M C Schotz, A S Garfinkel
  • PMID: 4641423
Free article

A simple lipase assay using trichloroacetic acid

M C Schotz et al. J Lipid Res. 1972 Nov.
Free article

Abstract

An extremely rapid and sensitive assay for lipoprotein lipase activity, suitable for routine determinations, is described. The substrate for the assay is emulsified [2-(3)H]glyceryl trioleate, activated by serum. The method is based on trichloroacetic acid precipitation of unreacted substrate and measurement of (3)H-labeled glycerol.

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