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. 1979 Mar;15(3):436-9.
doi: 10.1128/AAC.15.3.436.

Purification of a streptococcal bacteriocin (viridin B) and its separation from alpha-hemolysin

L D Apelgren, A S Dajani

Purification of a streptococcal bacteriocin (viridin B) and its separation from alpha-hemolysin

L D Apelgren et al. Antimicrob Agents Chemother. 1979 Mar.

Abstract

Viridin B, a bacteriocin produced by Streptococcus mitis, copurified with the alpha-hemolysin after ammonium sulfate precipitation and gel filtration on Sephadex G-200. The bacteriocin and hemolysin were separated in some instances by ion-exchange chromatography on diethylaminoethyl-Sephadex A-50, but the two substances were shown to be distinct after polyacrylamide gel electrophoresis. Attempts at recovery of nonhemolytic or nonbacteriocinogenic mutants were unsuccessful after exposure to mutagenic agents. The molecular weight of viridin B was determined to be approximately 87,000.

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References

    1. J Bacteriol. 1963 Oct;86:708-12 - PubMed
    1. Biochem Biophys Res Commun. 1960 Dec;3:575-7 - PubMed
    1. J Biol Chem. 1951 Nov;193(1):265-75 - PubMed
    1. Antimicrob Agents Chemother. 1978 Mar;13(3):473-8 - PubMed
    1. Proc Soc Exp Biol Med. 1977 Sep;155(4):456-60 - PubMed

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