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. 1979 Jun;66(2):337-42.
doi: 10.1111/j.1476-5381.1979.tb13685.x.

A study of the muscarinic receptor by gel electrophoresis

N J Birdsall, A S Burgen, E C Hulme

A study of the muscarinic receptor by gel electrophoresis

N J Birdsall et al. Br J Pharmacol. 1979 Jun.

Abstract

1 Muscarinic receptors from the brain of rat, guinea-pig and frog have been labelled with tritiated propylbenzilylcholine mustard ([3H]-PrBCM). 2 After solubilisation and sodium dodecyl sulphate (SDS)-polycarylamide gel electrophoresis a single radiolabelled peak was seen which was completely suppressed when labelling was carried out in the presence of 10(-6) M atropine. The results suggest the presence of a single polypeptide chain with a molecular weight of similar to or approximately 80,000. 3 In the case of intestinal smooth muscle multiple labelled peaks were found. Precautions against proteolysis led to the identification of a major peak with a molecular weight in the region of 80,000.

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References

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    1. Mol Pharmacol. 1978 Sep;14(5):723-36 - PubMed
    1. Methods Enzymol. 1974;32:82-91 - PubMed
    1. J Biol Chem. 1972 Sep 25;247(18):5856-61 - PubMed
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