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. 1975;41(3):275-9.
doi: 10.1007/BF00497691.

The influence of fixation on staining of glycosaminoglycans in glial cells

M Lai, I A Lampert, P D Lewis

The influence of fixation on staining of glycosaminoglycans in glial cells

M Lai et al. Histochemistry. 1975.

Abstract

Cytoplasmic staining of glial cells by Alcian blue in 0.40 M magnesium chloride can be demonstrated in sections of brain tissue fixed in formol-acetic acid and in Bouin's solution. Phosphate-buffered aldehyde fixatives, whether at neutral or low pH, fail to preserve stainable material.

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