Standardization of lymphocyte transformation to phytohaemagglutinin

Abstract

In contrast to the million cells used by many current workers, careful analysis of lymphocyte transformation to phytohaemagglutinin determined optimum conditions as (i) 200,000 lymphocytes in 1 ml of culture fluid in 100 × 12-mm round-bottomed glass tubes (ii) phytohaemagglutinin at 1 μg/tube (iii) ³H-thymidine at 1 μCi/tube (iv) with a convenient incorporation time 4 hr (v) starting 44 hr after adding phytohaemagglutinin. It was then found 10% autologous serum was the optimum concentration in the culture fluid. The log–normal range of transformation indices for young adults was 27–93–255.