Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1973 Jul;133(3):573-84.
doi: 10.1042/bj1330573.

Covalent chromatography. Preparation of fully active papain from dried papaya latex

K Brocklehurst, J Carlsson, M P Kierstan, E M Crook

Covalent chromatography. Preparation of fully active papain from dried papaya latex

K Brocklehurst et al. Biochem J. 1973 Jul.

Abstract

1. A Sepharose-(glutathione-2-pyridyl disulphide) conjugate has been prepared. 2. Its use in a new type of chromatography, covalent chromatography by thiol-disulphide interchange, is described. 3. With this technique, papain containing 1 intact catalytic site [thiol with high reactivity towards 2,2'-dipyridyl disulphide (2-Py-S-S-2-Py) at pH4] per mol of protein is readily prepared both from dried papaya latex and from commercial 2xcrystallized partially active papain. 4. The catalysis of the hydrolysis of alpha-N-benzoyl-l-arginine ethyl ester at pH6.0, 25.0 degrees C, I=0.3 by fully active papain thus prepared is characterized by K(m)=18.2+/-<0.1mm and k(cat.)=16.4+/-0.5s(-1).

PubMed Disclaimer

References

    1. FEBS Lett. 1970 Jul 29;9(2):113-116 - PubMed
    1. J Biol Chem. 1970 Jun;245(12):3059-65 - PubMed
    1. Biochem Biophys Res Commun. 1969 Mar 10;34(5):575-81 - PubMed
    1. Biochem J. 1972 Jul;128(4):811-6 - PubMed
    1. J Biol Chem. 1958 Dec;233(6):1387-91 - PubMed