[Studies of sorption and interaction of chlorophyll and chlorophyllase on methylaerosil]

Abstract

The article deals with the possibility of chlorophyll and chlorophyllase immobilization on methylaerosil and also with the effect of this carrier on the enzyme-substrate interaction. Chlorophyll and chlorophyllase are shown to be able to hydrophorbic interaction. It is established that the ultimate saturation of methylaerosil with chlorophyll is 16.1 mg per 100 mg of the carrier. The chlorophyllase reaction is the most active under conditions when the enzyme and substrate are in soluble states. The hydropholic binding of chlorophyll with methylaerosil decreases the intensity of its hydrolysis for the period of reaction and the preliminary immobilization of chlorophyllase on methylaerosil inhibits the reaction to a still greater extent. This evidences for the significance of the reaction system organization for interaction of chlorophyllase with chlorophyll.