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. 1973 Jul;134(3):817-20.

Regulation of vitamin D metabolism: factors influencing the rate of formation of 1,25-dihydroxycholecalciferol by kidney homogenates

K W Colston, I M Evans, L Galante, I MacIntyre, D W Moss

Regulation of vitamin D metabolism: factors influencing the rate of formation of 1,25-dihydroxycholecalciferol by kidney homogenates

K W Colston et al. Biochem J. 1973 Jul.

Abstract

The formation of 1,25-dihydroxycholecalciferol from 25-hydroxycholecalciferol by chick kidney homogenates is inhibited by increasing concentrations of Ca(2+). The apparent K(m) for the hydroxylation reaction is 1x10(-7)m, significantly lower than that reported for isolated mitochondria. Separated cytoplasmic and particulate fractions are inactive, but on recombination activity is restored, possibly because of the presence in the soluble fraction of a factor with a high affinity for 25-hydroxycholecalciferol.

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References

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