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Comparative Study
. 1973 Dec;26(6):863-6.
doi: 10.1128/am.26.6.863-866.1973.

Screening plate method for detection of bacterial beta-glucuronidase

G Dahlén, A Linde
Comparative Study

Screening plate method for detection of bacterial beta-glucuronidase

G Dahlén et al. Appl Microbiol. 1973 Dec.

Abstract

For screening tests of bacterial beta-glucuronidase (EC 3.2.1.31) activity an agar plate (MUG plate) containing the enzyme substrate, 4-methylumbelliferyl-beta-D-glucuronide, was developed. In the presence of beta-glucuronidase, the strongly fluorescent 4-methylumbelliferone, which can be detected by ultraviolet light, was liberated. For tests of fastidious bacteria, the MUG-plate was enriched with supplements containing heat-labile growth factors without influencing the reaction. The plate was found to be suitable for screening tests. The sensitivity of the MUG-plate was determined by means of fluorimetric method. With the present method, beta-glucuronidase activities were demonstrated in some well-known beta-glucuronidae-producing microorganisms as well as in Bacteroides and Corynebacterium spp.

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References

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