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. 1973 Sep;135(1):253-5.
doi: 10.1042/bj1350253.

The binding of cationic detergent to insulin, zinc-insulin and iso-insulin

K S Birdi

The binding of cationic detergent to insulin, zinc-insulin and iso-insulin

K S Birdi. Biochem J. 1973 Sep.

Abstract

Insulin, zinc-insulin and iso-insulin were precipitated by cetyltrimethylammonium bromide when the detergent/protein molar ratio was less than 10:1, whereas no precipitation was observed at ratios greater than 10:1. It is concluded that the detergent binds as a dimer at each binding site at ratios greater than 10:1. U.v. difference spectra indicated that the tyrosine residues were perturbed by detergent binding.

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