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. 1979 Jun;15(6):409-14.
doi: 10.1007/BF02618408.

Selection of a chemically defined medium for culturing adult newt forelimb regenerates

M E Conn, G E Dearlove, M H Dresden

Selection of a chemically defined medium for culturing adult newt forelimb regenerates

M E Conn et al. In Vitro. 1979 Jun.

Abstract

Three commercially available tissue-culture media were evaluated for their ability to support continued growth and differentiation of 14-day regenerates of adult newt forelimbs. Serums, embryo extracts, egg ultrafiltrates, and antimicrobial agents were avoided in this analysis. The hormones insulin and L-thyroxine were added to these chemically defined media to enhance continued cellular metabolism and growth. The optimum conditions appeared to be cultivated at 25 degrees C (pH 7.2 to 7.4) in media osmotically adjusted to conditions approximating amphibian blood values (i.e. 225 m0SM for 199, 244 m0SM for CMRL-1066, and 262 m0SM FOR L-15).

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