Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1979 Jul;62(1):109-12.
doi: 10.1007/BF00537012.

The kinetics of the histochemical method for dihydrofolate dehydrogenase (dihydrofolate reductase)

D Onicescu, V Atanasiu

The kinetics of the histochemical method for dihydrofolate dehydrogenase (dihydrofolate reductase)

D Onicescu et al. Histochemistry. 1979 Jul.

Abstract

The purpose of the present investigation is to report some histochemical and cytospectrophotometric observations providng more objective evidence for the specific activity of the histochemical method for dihydrofolate reductase activity (5.6.7.8-tetrahydrofolate: E.C. 1.5.1.3). Kinetic factors of the enzymic reaction, such as fixation, pH, coenzymes, substrate concentration, Michaelis constant, temperature, time of incubation, aerobiosis, and anaerobiosis, activators and inhibitors, were investigated.

PubMed Disclaimer

References

    1. Z Zellforsch Mikrosk Anat. 1971;117(4):463-75 - PubMed
    1. Histochem J. 1972 Mar;4(2):87-90 - PubMed
    1. Br J Haematol. 1974 Oct;28(2):199-208 - PubMed
    1. J Biol Chem. 1968 Nov 25;243(22):6001-8 - PubMed
    1. Histochem J. 1970 Jul;2(4):289-95 - PubMed

Substances