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Comparative Study
. 1979 Sep;139(3):984-92.
doi: 10.1128/jb.139.3.984-992.1979.

High-frequency fusion of Streptomyces parvulus or Streptomyces antibioticus protoplasts induced by polyethylene glycol

K Ochi, M J Hitchcock, E Katz
Comparative Study

High-frequency fusion of Streptomyces parvulus or Streptomyces antibioticus protoplasts induced by polyethylene glycol

K Ochi et al. J Bacteriol. 1979 Sep.

Abstract

Conditions were established for the regeneration of protoplasts of Streptomyces parvulus and Streptomyces antibioticus to the mycelial form. Regeneration was accomplished with a hypertonic medium that contained sucrose, CaCl2, MgCl2, and low levels of phosphate. High-frequency fusion of protoplasts derived from auxotrophic strains of S. parvulus or S. antibioticus was induced by polyethylene glycol 4,000 (42%, wt/vol). The frequency of genetic transfer by the fusogenic procedure varied with the auxotrophic strains examined. Fusion with auxotrophic strains of S. parvulus resulted in the formation of true prototrophic recombinants. Similar studies with S. antibioticus revealed that both stable prototrophic recombinants and heterokaryons were formed.

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References

    1. Biochem J. 1959 Nov;73:458-65 - PubMed
    1. J Bacteriol. 1964 Jun;87:1281-6 - PubMed
    1. J Gen Microbiol. 1963 Feb;30:249-60 - PubMed
    1. J Bacteriol. 1952 Oct;64(4):489-99 - PubMed
    1. J Bacteriol. 1977 May;130(2):946-7 - PubMed

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