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. 1974 Apr;27(4):671-3.
doi: 10.1128/am.27.4.671-673.1974.

Preparation of free heat-resistant ascospores from Byssochlamys asci

H D Michener, A D King Jr

Preparation of free heat-resistant ascospores from Byssochlamys asci

H D Michener et al. Appl Microbiol. 1974 Apr.

Abstract

When Byssochlamys is grown for production of ascospores, some of the asci break up into their constituent ascospores, whereas others do not. For heat resistance studies, it is desirable to prepare a uniform suspension of free ascospores. This was accomplished with the aid of a pressure cell, from which a suspension of asci under high pressure was released to atmospheric pressure through a small orifice. Spores so treated had about the same heat resistance as untreated spores.

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References

    1. Appl Microbiol. 1969 Jun;17(6):925 - PubMed
    1. J Bacteriol. 1969 Dec;100(3):1426-7 - PubMed
    1. Appl Microbiol. 1969 Aug;18(2):166-73 - PubMed

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